3′-End processing of pre-mRNA in eukaryotes

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Molecular mechanisms of eukaryotic pre-mRNA 3′ end processing regulation

Messenger RNA (mRNA) 3' end formation is a nuclear process through which all eukaryotic primary transcripts are endonucleolytically cleaved and most of them acquire a poly(A) tail. This process, which consists in the recognition of defined poly(A) signals of the pre-mRNAs by a large cleavage/polyadenylation machinery, plays a critical role in gene expression. Indeed, the poly(A) tail of a matur...

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Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export.

Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF I(m)68, stimulates mRNA export. CF I(m...

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Structure and Function of Pre-mRNA 5′-End Capping Quality Control and 3′-End Processing

Messenger RNA precursors (pre-mRNAs) are produced as the nascent transcripts of RNA polymerase II (Pol II) in eukaryotes and must undergo extensive maturational processing, including 5'-end capping, splicing, and 3'-end cleavage and polyadenylation. This review will summarize the structural and functional information reported over the past few years on the large machinery required for the 3'-en...

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Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3′-end processing

New transcripts generated by RNA polymerase II (RNAPII) are generally processed in order to form mature mRNAs. Two key processing steps include a precise cleavage within the 3' end of the pre-mRNA, and the subsequent polymerization of adenosines to produce the poly(A) tail. In yeast, these two functions are performed by a large multi-subunit complex that includes the Cleavage Factor IA (CF IA)....

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ژورنال

عنوان ژورنال: FEMS Microbiology Reviews

سال: 1999

ISSN: 1574-6976

DOI: 10.1111/j.1574-6976.1999.tb00400.x